The High-Throughput Screening Laboratory is a fee for service facility of University of Kansas, Lawrence and KU Medical Center that strives to provide researchers with the ability to run high-throughput chemical, siRNA, and high-content screens (HCS). The center incorporates compound libraries, instrumentation, databases and personnel.

The KU-HTSL is available for use by academic and private researchers to conduct biochemical and cell-based high throughput screens (HTS) to discover the effects of small molecules on targets of choice. The KU-HTSL can facilitate screening compound libraries in 96, 384 and 1536 well formats for both enzyme/protein and cell-based assays using industry-standard instrumentation and screening facilities.

Screening Facility

The HTS screening center is located in Structural Biology Center (SBC)-III complex on Lawrence campus. For directions, see Contact page. The screening center offers the following services:

  1. Discussion on screening goals, assays and instrument compatibility.
  2. Primary assay optimization and data analysis including protocols and selection of controls.
  3. Validation screening of a small library with a robust optimized assay.
  4. Screening the complete library of compounds.
  5. Data analysis and storage.
  6. Secondary screen development and/or screening of primary hits.
  7. Plating various dilutions of compounds and siRNA libraries.

The Director of KU-HTSL can provide letters of recommendation for those seeking financial support to defray the costs associated with the screening of small-molecule library. We can provide information about the compounds and instruments for grant submission. We can also assist in making your grant more competitive by generating some preliminary results using optimized assay to screen a small subset of our library collection.