The High-Throughput Screening Laboratory is a fee for service facility of University of Kansas, Lawrence and KU Medical Center that strives to provide researchers with the ability to run high-throughput chemical, siRNA, and high-content imaging screens (HCS). The center incorporates compound libraries, instrumentation, databases and personnel. We work on developing assays and executing screens encompassing a wide areas of research including cancer, infectious diseases, inflammation, neurological disorders as well as bioprospection and bioremediation.
The KU-HTSL is available for use by academic and private researchers to conduct biochemical and cell-based high throughput screens (HTS) to discover the effects of small molecules modulating targets of choice. The KU-HTSL can facilitate screening compound libraries in 96, 384 and 1536 well formats for both enzyme/protein and cell-based assays using industry-standard instrumentation and screening facilities. KU-HTS has the capability of developing assays using all available platform technologies (absorbance, fluorescence intensity, fluorescence polarization, FRET, TR-FRET, HTRF, luminescence, Alphascreen). We develop and optimize in vitro enzymatic assays (end-point, kinetic), protein binding assays (differential scanning fluorimetry), protein-protein, protein-nucleic acids binding assays, cell viability, reporter gene and image based phenotypic screening assays. We also have the capability to execute fast kinetic real-time screens for biochemical (e.g peptidyl-propyl cis-trans isomerases) and cell based GPCR/ion channel assays (e.g calcium mobilization) using the Hamamatsu FDSS system.
The KU-HTS screening center offers the following services:
- Discussion on screening goals, assays and instrument compatibility.
- Primary assay development and optimization.Selection of protocols and controls.
- Validation screening of a small library with a robust optimized assay.
- Screening the complete library of compounds (single or multiple compound concentrations, drug combinations).
- Data analysis and storage.
- Secondary screen development and/or screening of primary hits.
- Plating various dilutions of compounds and siRNA libraries.
- Hit to Lead optimization screens.
The Director of KU-HTSL can provide letters of recommendation for those seeking financial support to defray the costs associated with the screening of small-molecule library. We can provide information about the compounds and instruments for grant submission. We can also assist in making your grant more competitive by generating some preliminary results using optimized assay to screen a small subset of our library collection.